Ark and Culture Biosciences demonstrated what becomes possible when adaptive digital twins are connected directly to API-native bioreactors. In a recent collaboration, Ark’s platform used historical run data, real-time bioreactor data, and daily offline measurements to continuously retrain its model and update the feed strategy during the run. The result: the top digital twin condition delivered a 43% titer improvement over the historical best run, showing how closed-loop optimization can move bioprocessing beyond static recipes and toward adaptive, data-driven process development.